Advantages of Targeted Digital Cytometry for Cell Type Composition Profiling
Current methods for determining cell type composition have limitations including throughput, cost, and artifacts introduced by tissue dissociation. Thus, deconvolution algorithms for determining cell type composition from bulk tissue expression profiles have gained traction.
CiberMed’s iSort™ is a digital cytometry solution based on CIBERSORTx with novel enhancements. Pairing iSort with Agilent SureSelect targeted sequencing enables a new approach for “targeted digital cytometry,” which achieves highly accurate and reproducible enumeration of cell subsets in blood and solid tissue samples, while reducing the sequencing requirement over whole transcriptome profiling by up to 50-fold.
This webinar will present two NGS target enrichment panels optimized for determining cell type composition from both blood and tissue samples, discuss the gene signature matrices used to inform these panels, show data demonstrating the above performance gains, and conclude with application-specific use cases where determining cell type composition by deconvolution has demonstrated advantages over more traditional methods such as flow cytometry or single cell RNA sequencing.
Key learning objectives
- Understand how targeted digital cytometry addresses limitations of traditional methods for determining cell type composition
- Learn how novel enhancements in CiberMed’s iSort™ software, paired with Agilent SureSelect targeted sequencing panels, improve the accuracy and reproducibility of cell subset enumeration
- Explore the scalability and performance gains of targeted digital cytometry
Who should attend?
- Immunology, oncology, molecular biology researchers – interested in advanced methods for cell type composition analysis
- Bioinformaticians - implementing deconvolution algorithms
- Clinical lab professionals – seeking methods to improve cell profiling within FFPE samples
- Pharma and biotech industry professionals – applying cell profiling to biomarker discovery and drug development
- Academics – interested in advancements and applications for targeted digital cytometry
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
Speakers
Moderator
