Advancement of analytical technology for oligonucleotide analysis using high-resolution accurate-mass mass spectrometry
The advancement of oligonucleotide therapeutics and mRNA vaccines drives the need to develop robust analytical approaches for confident identification and in-depth characterization of these novel modalities.
Dr. Kevin Kun Guo, senior applications scientist and Keeley Murphy, software product manager, Thermo Fisher Scientific, will present the latest development of high-resolution accurate-mass (HRAM) based LC-MS methods for oligonucleotide and RNA analysis. They will also describe a data dependent MS/MS (ddMS2) method for high confidence identification, mapping, and relative quantitation of oligonucleotides and their impurities within a single experiment.
Plus, the capability of the Thermo Scientific™ Vanquish Horizon™ UHPLC system and DNAPac™ RP column for robust and reproducible separation of oligonucleotides under the denaturing and non-denaturing conditions will be demonstrated.
Finally, the speakers will explore the power of the Thermo Scientific™ Orbitrap Exploris™ mass spectrometers for accurate mass measurement of oligonucleotides as large as 100mer.
Key learning objectives
- Discover new LC-MS methods for oligonucleotide and RNA analysis
- Understand how a data dependent MS/MS (ddMS2) method can be applied to oligonucleotide identification, mapping, relative quantitation and impurity analysis
- Find out how BioPharma Finder™ Software can support your oligonucleotide analysis
Who should attend?
- Biopharmaceutical research scientists
- Analytical scientists from biopharma companies working on or interested in oligonucleotide based therapies
- PhD students in any field of biotherapeutic drug development
- Scientists looking to advance knowledge of oligonucleotide analysis
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
Speakers
Moderator
