Cell line engineering with CRISPR-Cas9: Cell line optimization

21 October 2020

Emily Hardy, Manager of Cell Engineering Services at Horizon Discovery, discusses cell line optiization techniques including what reagent formats you can use for both guide RNA and Cas9 expression, methods to optimize transfection efficiencies for those crispr reagents and key consideratons for downstream analysis, such as clonal isolation and cell culture homogeniety.