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Fast-Acquisition Breath Analysis Configuration

Agilent’s Low Input Quick Amp WT Labeling Kit reliably amplifies and labels whole transcripts for eukaryote and prokaryote samples, with a low sample input range of 25-100 ng total RNA.

The Agilent Model Organism CGH Microarray is a dual color array containing 60-mer oligonucleotide probes synthesized in situ using Agilent’s inkjet SurePrint technology. It provides comprehensive coverage of the model organisms genome enabling genome-wide DNA variation profiling.

Agilent’s proprietary Stabilization and Drying Solution is optimized to reduce degradation of cyanine 3 and 5 by ozone during microarray processing in preparation for scanning. It should be used when the ozone level in the microarray processing area is higher than 10 ppb.

The pET E. coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.

The Complete Control Inducible Mammalian Expression System is designed to provide the tightest control of expression available in mammalian cells. The system is based on a synthetic ecdysone-inducible receptor and a synthetic receptor recognition element that modulates expression of your gene of interest.  

The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in vivo is achieved via transient expression in a variety of cell lines (highest level of expression is obtained following transfection of a cell line expressing the T antigen).

AffinityScript Multiple Temperature Reverse Transcriptase (RT) produces high cDNA yields over the broadest temperature range with high affinity to primer/template complexes. It delivers high cDNA yield when amplifying low RNA input amounts and resolving RNA secondary structure using temperatures up to 55°C.

Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations. For faster experiments with earlier Ct's and greater reproducibility, Agilent recommends Brilliant III Ultra-Fast master mixes.

Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations. For faster experiments with earlier Ct's and greater reproducibility, Agilent recommends Brilliant III Ultra-Fast master mixes.

The High Sensitivity DNA kit allows analysis of fragmented DNA or DNA libraries.

Easy-A Hi-Fi Cloning Enzyme & Master Mix combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.