Mesenchymal stem cell cultivation in single-use stirred tank bioreactors

Mesenchymal stem cells (MSCs) are a relatively rare cell population, and large quantities of cells are typically required for clinical applications. This creates the need for large-scale platforms to efficiently expand MSCs. MSCs have gained significant attention because of their therapeutic potential for many difficult-to-treat diseases. In this application note, PromoCell describes an easy-to-understand protocol for the Good Manufacturing Practice (GMP)-compliant large-scale production of MSCs in a single-use bioreactor. The note also includes results regarding attachment and cell expansion, and results of the metabolite analysis.