ResourceSeparations

HPLC: Improved sample preparation for high-sensitivity analysis

Save time and increase reproducibility with advanced HPLC sample quality control and testing capabilities

14 Nov 2019

High-performance liquid chromatography (HPLC) is a powerful analytical tool used for the separation, identification and quantification of individual compounds within a sample.

Using high-pressure systems and the latest column chemistry, this technique makes separations faster, reduces solvent consumption and increases resolution and sensitivity of results. Effective sample and standards preparation are important steps in order to achieve reproducible and specific results.

In this application-based eBook, we look at effective HPLC sample preparation and cover:

  • Preparation of solvents
  • Preparation of standards
  • Filtration systems

Claristep® filtration system

Sartorius Group

Claristep® is a filtration system designed to save you both time and effort. Thanks to the patented design of the Claristep® station, you can now quickly and easily filter up to eight 60 μL to 600 μL HPLC samples in parallel using one hand – without the need for AC power, vacuum pumps or syringes.

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Arium® Pro Type I Ultra Pure Water Systems

Sartorius Group

Arium® Pro Type I ultra pure water systems for the production of reagent-grade water needed for critical applications as well as routine analyses. Typical applications are the production of ultrapure water for cell culture or chromatographic applications. The Type I ultrapure water systems are available in four different versions and can be ordered as wall-mounted unit, bench-top system or built-in system. Arium® Pro features a convincing innovative design, quality and ease of use. Sophisticated display and menu functions User can choose between three Comfortable water dispense setups Highly developed electronic features Display and menu functions The innovative glass operator interface with touch function can be operated easily. The intuitive menu navigation with integrated text support is easy to handle. Pre-setting of relevant parameters for documentation, e.g. conductivity feed and product PIN protected menu and service setting Service and alarm functions supported by graphic presentation Color-coded warnings Displays measured values for water (feed water, product water, temperature, total dispended water), system information and count down for next recommended consumable replacement. Integrated totalizer to monitor water consumption Diary function Water Dispense With the manual, volume and time dispensed water control the Arium® pro gives users the greatest possible flexibility in routine applications. A countdown for the remaining amount of water is displayed and the system comes with a volume and time memory function. Manual dispense (stepless from 0,5 up to 2 l/min) Volume dispense (0,1 – 60 l) Time dispense (30 sec – 59 min) Electronic features for data backup and display Arium® Pro automatically measures the conductivity of feed and product water. These and other parameters can be displayed continuously or directly on a PC or printer and can be saved on a SD card as well. Related products Arium Smart Station Arium® Mini

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HPLC: Improved sample preparation for high-sensitivity analysis