Cell based assays: The optimal reads function for fluorescence bottom measurements

19 Feb 2024

In this application note, Tecan highlights the Optimal Read (OR) function for bottom reading fluorescence intensity measurements in adherent cell lines. Performing single fluorescence reads in the center of the well can give a false impression of the cell density in cases where the cells show non-uniform distribution across the growth surface. The OR function for fluorescence bottom measurements is designed to enable uniform well illumination during excitation with whole-well emission, ensuring that even heterogeneous cell layers can be reliably analyzed. The note provides detailed information on the experimental setup, including measurement settings, and presents results showing improved uniformity and reproducibility of well-to-well measurements.