Calcium Signaling with FLIPR Calcium 6 and 6-QF Assay Kits on the FlexStation 3 Reader

12 Feb 2019

FLIPR® Calcium Assay Kits from Molecular Devices employ sensitive calcium indicators and proprietary masking dyes to enable researchers to conduct highly sensitive fluorescent screens of G-protein coupled receptors (GPCRs), ion channels, and other calcium sensitive targets. By using a novel dye formulation to further enhance the calcium flux assay signal window, assay robustness is increased, while providing greater assay protocol flexibility. As a result, the FLIPR Calcium 6 and Calcium 6-QF Calcium Kit dyes become more suitable for measuring calcium flux in 384-well plates with the medium throughput FlexStation® 3 MultiMode Microplate Reader using the 16 channel pipettor. Results are similar to those obtained on the FLIPR® Tetra System in high throughput mode.

SoftMax® Pro Microplate Data Acquisition & Analysis Software

Molecular Devices®

SoftMax ® Pro Software is designed to provide the simplicity, flexibility and power required for advanced data analysis. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Every step has been optimized for data acquired from a Molecular Devices microplate reader or data imported from another source to simplify analysis and reporting. FDA 21 CFR Part 11 compliance tools are available for regulated laboratories providing end-to-end chain of custody.

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