Automated generation of clonal hiPSC cultures

Human induced pluripotent stem cells (hiPSCs), in combination with powerful gene-editing technologies, such as CRISPR-Cas9, offer unique possibilities to generate sophisticated disease models and advance drug discovery. However, the process of generating gene-edited hiPSC lines is often tedious and time-consuming, with the generation of clonal hiPSC lines from a heterogenous pool of edited cells representing a critical bottleneck for setting up an efficient pipeline. iotaSciences explores how the automated Cloning Platform facilitates gentle single-cell cloning, offering documented proof of monoclonality and greatly reducing reagent usage.