Advancements in Spectral Flow Cytometry Instrumentation
19 Feb 2019This application note provides relevant information on the current instrumentation involved in combining spectography and flow cytometry. Spectral flow cytometry is a technique based on conventional flow cytometry where a spectrograph and multichannel detector (usually CCD) is substituted for the traditional mirrors, optical filters and photomultiplier tubes (PMT) in conventional systems.
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Oxford Instruments AndorThe Newton CCD camera is ideally suited to high-performance spectroscopic applications. The high-resolution sensors deliver up to 95% QE. Combined with Andor Technology’s low noise electronics, it provides convenient multi-Megahertz readout and USB connectivity. The camera utilizes a 1024 x 255 array of 26μm² pixels with thermoelectric cooling down to -100°C resulting in negligible dark current. The system offers the best performance for a wide range of spectroscopic applications across VUV-NIR wavelengths.