A Comparison of Two Solid Phase Extraction Methods for the Analysis of Polyaromatic Hydrocarbons in Butter

Application: The 35% phenyl equivalent phase provides a higher polarity option compared to columns containing a lower phenyl content, resulting in a greater retention of polar analytes relative to non-polar compounds. Its selectivity is complementary when paired with SLB-5ms for applications that require dual-column confirmatory analysis, such as for environmental analytes (such as pesticides, PCBs, and herbicides). The low bleed characteristics, inertness, and durable nature make it a great column anywhere a low bleed intermediate polar column is required.   USP Code: This column meets USP G42 requirements.   Phase: Bonded and highly crosslinked; proprietary polymer virtually equivalent in polarity to poly(35% diphenyl/65% dimethyl siloxane)   Temp. Limits: ≤0.32 mm I.D.: ambient to 350 °C (isothermal) or 360 °C (programmed)   Temp. Limits: ≥0.53 mm I.D.: ambient to 330 °C (isothermal) or 340 °C (programmed)

This multi-bed solid phase extraction (SPE) cartridge was designed for the extraction of non-polar persistent organic pollutants (POPs), specifically heavy and light polynuclear aromatic hydrocarbons (PAHs), from edible oil matrices such as olive and corn oil. Fatty matrix is preferentially retained by the cartridge while PAHs are washed through using acetonitrile. The resulting extract is suitable for either GC-MS or HPLC-FLD analysis. The top layer of the SPE cartridge consists of Florisil®, a polar adsorption media that interacts with polar functional groups such as acids and alcohols. The bottom layer is comprised of a mixture of Discovery® DSC-18 and Z-Sep sorbents. C18 binds fatty matrix through hydrophobic interaction. Z-Sep, a zirconia coated silica, acts as a Lewis acid, interacting with the oxygen within OH and COOH groups found in fatty acids and mono and diacylglycerols.Features: Dual layer SPE that contains both LC-Florisil® (upper layer) and Z-Sep/C18 (lower layer) SPE sorbents, separated by a PE frit. Developed to offer superior cleanup when analyzing non-polar persistent organic pollutants (POPs) from oil matrices. Fatty matrix is preferentially retained by the cartridge, while non-polar POPs are washed through using acetonitrile.