Transfection Reagents and Equipment Products & Reviews

Developed through years of experience with the Flaming/Brown style micropipette pullers and infused with leading edge technology, Sutter is proud to introduce the P-1000 micropipette puller. The most obvious new feature is the color touchscreen display that provides an intuitive and full-featured interface. Key Features: Color touch-screen interface Safe heat mode to protect and extend filament life Line repeat mode sim…

A significant advance in the technology of fabrication of micropipettes, optical fiber probes, and nanospray tips, is offered with the P-2000 micropipette puller. The P-2000 integrates a CO2 laser-based heat source with the technology derived from our extensive experience with conventional pullers. This system offers capabilities unmatched by other pullers. Key Features: Capable of pulling quartz, borosilicate and alumino…

High productivityVersatile: efficient in both HEK 293 & CHO synthetic mediaComplies with new regulatory requirements for large scale production

Reliable Transfection for Common Cell Lines

The GeneJammer Transfection Reagent is a proprietary formulation of novel polyamines ideal for transient or stable transfections in a wide variety of cell types. This transfection reagent is effective in both serum-containing medium and serum-free medium, offering high efficiencies with minimal cytotoxicity.

The LipoTAXI mammalian transfection reagent contains a unique, low-toxicity lipid formulation that has been tested with over 30 cell lines and has been shown to achieve high efficiency transfections with a wide variety of cells. Compared to CaPO4/DEAE-mediated transfection, it provides a 10-fold increase in transient transfection efficiency in many cell lines. This transfection reagent may be used with both adherent cells and…

The MBS Mammalian Transfection kit can increase your transfection efficiencies up to 100-fold. Cells are incubated with CaPO 4 /DNA coprecipitate for only 3 hours, versus 6 to 12 hours with traditional CaPO 4 methods. Additionally, cells can be assayed 12 to 24 hours post-transfection instead of the usual 48 to 72 hours.  

The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. 

The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. 

The SatisFection reagent is a novel cationic polymer suitable for both transient and stable transfections in the presence or absence of serum. This polymer functions by binding and condensing DNA into a polyplex that enters the cell via endocytosis.  Cost savings of up to 70% per transfection  Unmatched performance in some hard-to-transfect lines  Maximum expression 36 - 72 hours post-transfection  Ideal reagent for exp…

The SatisFection reagent is a novel cationic polymer suitable for both transient and stable transfections in the presence or absence of serum. This polymer functions by binding and condensing DNA into a polyplex that enters the cell via endocytosis.  Cost savings of up to 70% per transfection  Unmatched performance in some hard-to-transfect lines  Maximum expression 36 - 72 hours post-transfection  Ideal reagent for exp…

The Big Blue Lambda LIZ (LacI/Z) shuttle vector is the bacteriophage lambda vector used in the Big Blue transgenic rodent mutation assay. The utility of this vector is its ability to act as a target for mutations in vivo that can subsequently be shuttled to E.coli for analysis.  Ideal shuttle vector for any plaque assay  Suitable for standard CaPO4 transformations  Available to researchers for transfection of existing cel…

The Complete Control Inducible Mammalian Expression System provides the tightest control of expression available in mammalian cells. 

The ViraPack Transfection Kit is based on a modified CaPO4 method of DNA transfection to introduce viral plasmids into HEK293 packaging cell lines with high efficiency.

Helper phages for mass excision of Lambda ZAP, ZAP Express libraries, and for single strand rescue protocols.  Use for mass excision of Lambda ZAP®, and ZAP Express® libraries  Interference-resistant helper phage  Eliminates the possibility of co-infection from helper phage

The ExAssist helper phage with XOLR works with the Lambda expression vector system kits for mass excision of libraries in ZAP Express or Lambda ZAP vectors. The pCMV-Script or pBluescript phagemid will grow.

Agilent provides a range of Interference-resistant Helper Phages that enable ssDNA recovery from phagemid vectors. The pBlueScript II Phagemid Vector systems are available in R408 and VCSM13 format. R408 Interference-Resistant Helper Phages are stable and easy to grow, and are provided in a single-strand size of ~4 KB.

Helper phages for mass excision of Lambda ZAP, ZAP Express libraries, and for single strand rescue protocols.  Use for mass excision of Lambda ZAP®, and ZAP Express® libraries  Interference-resistant helper phage  Eliminates the possibility of co-infection from helper phage

Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.

Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.

The MBS Mammalian Transfection Kit increases transfection efficiencies by up to 100-fold. Cells are incubated with CaPO4/DNA coprecipitate for only 3 hours compared to 6 to 12 hours with traditional CaPO4 methods. 

The In Situ beta-Galactosidase Staining Kit provides an easy and rapid method to determine transfection efficiency by indicating beta-Galactosidase activity in individual intact cells.  In situ staining of cells directly in dishes without removing or manipulating cells  Rapid detection of Ÿ-gal activity in transfected cells  Results in 24 hours  Maximum signal with low background  Deep blue staining reaction  Kit in…

Modulation of gene expression with modified oligos

The Mammalian Cell Transfection Kit is an optimized system suitable for the introduction of DNA into mammalian cells. DNA is introduced to the cells as a calcium phosphate precipitate. Transfected cells integrate and express the exogenous DNA at a higher efficiency, producing stable clones of the specifically altered genotype and phenotype. This system has been used to introduce various plasmids into a broad range of cell line…

A highly efficient method of gene trasfer into mammalian cells is through infection with retroviral vectors. The efficiency of retroviral infection is enhanced significantly, 100 to 1,000 fold in some cells, by including polybrene during the infection. Polybrene with DMSO shock is also used to mediate DNA transfer into a variety of cell types, such as: CHO, chicken embryo fibroblases, NIH3T3 cells, and myeloid cells.