Drug discovery > Target Discovery Products & Reviews

The pCMV-Script PCR cloning kit is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives. In the ligation reaction, PCR products are incubated with the predigested pCMV-Script mammalian expression vector, the restriction enzyme Srf I, and T4 DNA ligase.  Cl…

BL21-Gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene encoding endonuclease I (endA) inactivated. 

The XL1-Blue strains are an all-prupose line of competent cells that are ideal for routine cloning needs. 

Most E. coli hosts contain both DNA adenine methylation (dam) and DNA cytosine methylation (dcm) genes. These genes code for proteins that methylate specific sequences when DNA is propagated, making subsequent digestion with methylation-sensitive restriction enzymes impossible. Agilent offers the SCS110 and JM110 strains, which lack both dam and dcm activity. 

This collection of competent cells provides customers with classic E. coli strains that have been engineered to become competent for use in cloning experiments.

High efficiency competent cells in convenient packaging designed to satisfy higher throughput requirements. For ultimate convenience we offer custom configurations, formulations, and packaging options.

High efficiency competent cells in convenient packaging designed to satisfy higher throughput requirements. For ultimate convenience we offer custom configurations, formulations, and packaging options.

Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems. The SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryoti…

Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems. The SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryoti…

Replicating eukaryotic DNA in prokaryotic cells can be problematic. Particular eukaryotic genes may contain inverted repeats or secondary structures, such as Z-DNA, that can be rearranged or deleted by E. coli DNA repair systems. The SURE competent cells are deficient in the E. coli genes involved in the rearrangement and deletion of DNA, thus improving cloning efficiencies of DNA containing irregular structures in prokaryoti…

Electroporation-competent cells capable of producing greater than 1 × 1010 transformants/µg of DNA. These electroporation-ready cells need only be thawed, mixed with DNA, and electroporated. TG1 electroporation-competent cells are recommended for preparation of phage display libraries.

TKB1 Competent Cells are derived from the BL21 strain and are intended for use with pET, pCAL or other T7 RNA promoter-based vectors. TKX1 Competent Cells are derived from the XL1-Blue strain and are intended for use with non-T7 promoter based vectors.

TKB1 Competent Cells are derived from the BL21 strain and are intended for use with pET, pCAL or other T7 RNA promoter-based vectors. TKX1 Competent Cells are derived from the XL1-Blue strain and are intended for use with non-T7 promoter based vectors.

These competent cells offer the highest transformation efficiencies available, enabling dramatically improved transformation efficiencies when cloning large plasmids and ligated DNA. 

These competent cells offer the highest transformation efficiencies available, enabling dramatically improved transformation efficiencies when cloning large plasmids and ligated DNA.

The XL1-Blue strains are an all-prupose line of competent cells that are ideal for routine cloning needs. 

The XL1-Blue strains are an all-prupose line of competent cells that are ideal for routine cloning needs. 

When DNA is methylated in a fashion unlike the bacterial host patterns, it is cleaved by the E. coli host restriction systems. 

When DNA is methylated in a fashion unlike the bacterial host patterns, it is cleaved by the E. coli host restriction systems. 

When DNA is methylated in a fashion unlike the bacterial host patterns, it is cleaved by the E. coli host restriction systems.

When DNA is methylated in a fashion unlike the bacterial host patterns, it is cleaved by the E. coli host restriction systems. 

When DNA is methylated in a fashion unlike the bacterial host patterns, it is cleaved by the E. coli host restriction systems. 

The XL1-Blue strains are an all-prupose line of competent cells that are ideal for routine cloning needs. 

The XL1-Blue strains are an all-prupose line of competent cells that are ideal for routine cloning needs.

These competent cells enable a highly efficient, rapid, and reproducible method for introducing random mutations in a cloned gene of interest. This method involves propagating the cloned gene into XL1-Red Competent Cells; an Escherichia coli strain which is deficient in three of the primary DNA repair pathways.