ZymoBIOMICS™ Microbial Community Standard by Zymo Research

Manufacturer Zymo Research  |   Model: D6300
4.5
/
5.0
  |  10 reviews
Assess bias and errors in NGS-based microbial composition profiling workflows


ZymoBIOMICS™ Microbial Community Standard by Zymo Research product image
ZymoBIOMICS Community Standard
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Average Rating: 4.5
10 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2021

  • Organization: Duisburg-Essen University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Exceptional product, reliably of high-quality!
Rating: 5.0

  • Application Area: Microbiology

"The ZymoBIOMICS Microbial Community Standard is a reliable resource that I have used to standardize microbial molecular biology protocols as well as benchmark bioinformatics tools. This standard has allowed colleagues in my laboratory in early levels of training to safely be introduced to specific techniques, as well as other members with more experience to develop and test their own new protocols. I'd recommend the ZymoBIOMICS Microbial Community Standard fo any laboratory in need of a standardized mock community for molecular biology and bioinformatics applications."

Review date: 07 Jun 2021 | ZymoBIOMICS™ Microbial Community Standard
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Oklahoma State University



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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Is the best microbial community standard I have used.
Rating: 4.3

  • Application Area:Microbiome studies

"The Zymobiomics Microbial Community standard is the go-to positive control for all the microbiome studies we do in our lab, based on 16S rRNA gene amplicon sequencing and metagenome sequencing. It consists of good combination of gram positive and negative bacteria, and fungal communities with known relative abundance values. Hence it is ideal to be used as a microbial community standard in experiments that depend on the efficacy of the DNA extraction methodology for reproducible results. A single vial of 750µl (which costs around $300) can be used on 10 different DNA extractions due to its low starting material requirement (75µl). Hence, it gives you high value for money and ease of use. Furthermore, it carries good documentation that clearly indicates what should be the expected relative abundances of the standard microbial communities, for microbial community reconstruction done using it. Thus making it easy to compare results, and pinpoint where certain biases may lie."

Review date: 16 Mar 2021 | ZymoBIOMICS™ Microbial Community Standard
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: Microba Pty Ltd



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product that is highly reliable and relevant.
Rating: 5.0

  • Application Area:Standardization of testing

"This product is reliable and of high quality. Batch variation is well controlled. Good mix of organisms mean it suits a variety of applications."

Review date: 16 Mar 2021 | ZymoBIOMICS™ Microbial Community Standard
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Dominican University of California



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good reliable standard.
Rating: 5.0

  • Application Area:Skin microbiome of salamanders

"The product worked great and it really helped increase my confidence in my results!"

Review date: 04 Dec 2020 | ZymoBIOMICS™ Microbial Community Standard
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of California



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Great positive control for microbial community work.
Rating: 4.3

  • Application Area:Research

"This product provided a solution to an issue we have had for a while, a positive control for microbial community work. They sell kits that have been extracted with notes so that you can see which step introduces contamination or error in your microbial community work (we have used it for 16S amplicon sequencing)."

Review date: 04 Dec 2020 | ZymoBIOMICS™ Microbial Community Standard
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Floragraph, Inc.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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A common sense cellular standard for microbiome research
Rating: 4.7

  • Application Area:Reference and control for microbiome studies

"A critical reagent for standardizing microbiome research. Exactly what we (and many others) need to QC steps in our processes and ensure a reproducible standard. This used to be shipped frozen and now ships at RT (in a stabilization buffer), which was initially alarming, but we haven't noticed significant differences."

Review date: 11 Dec 2019 | ZymoBIOMICS™ Microbial Community Standard
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Safs uq



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy, fast no hassles and better results.
Rating: 5.0

  • Application Area:Microbial community analysis of marine environments

"If you want a fast easy way to normalize your community profiling this is the best way! You will be really surprised with the results and how much it can change your results."

Review date: 28 Nov 2019 | ZymoBIOMICS™ Microbial Community Standard
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Oxford



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
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Product performs as described.
Rating: 4.3

  • Application Area:Control for experiments

"Reproducible results from product. Does what it says."

Review date: 27 Nov 2019 | ZymoBIOMICS™ Microbial Community Standard
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: The Walter and Eliza Hall Institute



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
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Very useful, I use it in every NGS sequencing run.
Rating: 3.7

  • Application Area:Evaluate DNA extractions for NGS

"Very easy to use and useful for tracking DNA extraction efficiency and as QC for each step in the NGS library workflow. Also handy for bioinformatic analyses as it makes comparisons between sequencing runs easier and helps to track the performance of the instrument."

Review date: 06 Jun 2019 | ZymoBIOMICS™ Microbial Community Standard
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Nabsys



  • Ease of use
    3 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Good standard but needs to prepared differently.
Rating: 4.0

  • Application Area:Strain identification

"We used the microbial community standard in a proof of concept experiment for strain identification. We isolated DNA from the mixture of cells but were unable to obtain long DNA lengths for the gram-negative bacteria. This is presumably because the sample was not treated gently after the addition of the DNA/RNA shield."

Review date: 05 Jun 2019 | ZymoBIOMICS™ Microbial Community Standard

ZymoBIOMICS™ Microbial Community Standard is a mock microbial community consisting of eight bacterial and two fungal strains. It includes three easy-to-lyse Gram-negative bacteria (e.g. Escherichia coli ), five tough-to-lyse Gram -positive bacteria (e.g. Listeria monocytogenes ), and two tough-to-lyse yeasts (e.g. Saccharomyces cerevisiae ). Seven of these strains are known human pathogens and have been fully inactivated with DNA/RNA Shield™. The GC content1of the contained genomes covers a range from 15% to 85%. It was constructed by pooling pure cultures of the ten microbial strains. The cells and DNA content of each pure culture were quantified before pooling. Cultures were mixed to a predeterminedcomposition. The actual microbial composition was measured using next-generation sequencing techniques after extracting the genomic DNA with the ZymoBIOMICS™ DNA Mini kit.

Product Overview

ZymoBIOMICS™ Microbial Community Standard by Zymo Research product image

ZymoBIOMICS™ Microbial Community Standard

Manufacturer Zymo Research

4.5 / 5.0 | 10 reviews