ZymoBIOMICS™ Microbial Community Standard by Zymo Research

Manufacturer Zymo Research  |   Model: D6300
4.3
/
5.0
  |  4 reviews
Assess bias and errors in NGS-based microbial composition profiling workflows


ZymoBIOMICS™ Microbial Community Standard by Zymo Research product image
ZymoBIOMICS Community Standard
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Average Rating: 4.3
4 Scientists have reviewed this product

4 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: Safs uq



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Easy, fast no hassles and better results.
Rating: 5.0

  • Application Area: Microbial community analysis of marine environments

"If you want a fast easy way to normalize your community profiling this is the best way! You will be really surprised with the results and how much it can change your results."

Review date: 28 Nov 2019 | ZymoBIOMICS™ Microbial Community Standard
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Oxford



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
Product performs as described.
Rating: 4.3

  • Application Area:Control for experiments

"Reproducible results from product. Does what it says."

Review date: 27 Nov 2019 | ZymoBIOMICS™ Microbial Community Standard
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: The Walter and Eliza Hall Institute



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Very useful, I use it in every NGS sequencing run.
Rating: 3.7

  • Application Area:Evaluate DNA extractions for NGS

"Very easy to use and useful for tracking DNA extraction efficiency and as QC for each step in the NGS library workflow. Also handy for bioinformatic analyses as it makes comparisons between sequencing runs easier and helps to track the performance of the instrument."

Review date: 06 Jun 2019 | ZymoBIOMICS™ Microbial Community Standard
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Nabsys



  • Ease of use
    3 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Good standard but needs to prepared differently.
Rating: 4.0

  • Application Area:Strain identification

"We used the microbial community standard in a proof of concept experiment for strain identification. We isolated DNA from the mixture of cells but were unable to obtain long DNA lengths for the gram-negative bacteria. This is presumably because the sample was not treated gently after the addition of the DNA/RNA shield."

Review date: 05 Jun 2019 | ZymoBIOMICS™ Microbial Community Standard

ZymoBIOMICS™ Microbial Community Standard is a mock microbial community consisting of eight bacterial and two fungal strains. It includes three easy-to-lyse Gram-negative bacteria (e.g. Escherichia coli ), five tough-to-lyse Gram -positive bacteria (e.g. Listeria monocytogenes ), and two tough-to-lyse yeasts (e.g. Saccharomyces cerevisiae ). Seven of these strains are known human pathogens and have been fully inactivated with DNA/RNA Shield™. The GC content1of the contained genomes covers a range from 15% to 85%. It was constructed by pooling pure cultures of the ten microbial strains. The cells and DNA content of each pure culture were quantified before pooling. Cultures were mixed to a predeterminedcomposition. The actual microbial composition was measured using next-generation sequencing techniques after extracting the genomic DNA with the ZymoBIOMICS™ DNA Mini kit.

Product Overview

ZymoBIOMICS™ Microbial Community Standard by Zymo Research product image

ZymoBIOMICS™ Microbial Community Standard

Manufacturer Zymo Research

4.3 / 5.0 | 4 reviews