Member since: 2015
Organization: Washington University in St Louis
Easy to use and good yield, but high contamination.
Application Area: Extract small extracellular vesicles/exosomes from plasma
"The kit is easy to use and readily allows the extraction of extracellular vesicles. However, there seems to be strong lipid contamination and I have confirmed the presence of clotting factors (i.e. fibrin) that can still clot if resuspending EVs in culture media with calcium. Overall, the yield is high, but the specificity is low with potentially problematic contaminants."
Member since: 2020
Organization: Northwestern University
Exosome RNA extraction.
Application Area:Exosome research
"Used this kit for total RNA extraction from exosomes. The yield is excellent, and the extracted RNA can be used for next-generation sequencing after quantifying with bioanalyzer."
The Total Exosome RNA and Protein Isolation Kit enables isolation of total RNA and proteins from exosome samples extracted from biological fluids (e.g., by using Total Exosome Isolation (from cell culture media) or Total Exosome Isolation (from serum)).
The Total Exosome RNA and Protein Isolation Kit is designed for the isolation of both RNA and protein from pre-isolated or enriched exosomes (not supplied). It is suitable for studies of RNA expression (specifically miRNA), processing, or function. The kit allows recovery of both RNA and proteins from the same purified exosome preparation. One portion of the sample is subjected to organic extraction, followed by immobilization of RNA on glass-fiber filters to purify total RNA or enrich for small RNA species. The other portion is then used directly for common protein applications such as Western blotting.
High yields of ultra-pure RNA can be prepared in about 30–60 minutes. The RNA can then be used in a variety of downstream applications, including qRT-PCR, high-throughput sequencing (e.g., using the Ion Torent™ PGM™, Ion Torrent™ Proton™, or SOLiD® sequencing instruments), microarray analysis, solution hybridization assays, and blot hybridization.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.