Member since: 2020
Organization: University of Basel
Simple and efficient.
Application Area: Transport of solutes and derivatives via proteoliposomes
"Very easy to use and the results are highly reproducible. Requires only a fraction of sample compared to other methods and several sets of measurements can be performed in only one day."
The SURFE²R N1 works with reusable sensor chips on which the membranes containing the protein of interest are adsorbed. In contrast to conventional patch-clamp electrophysiology the proteins of electrogenic transporters and pumps have low turnover rates compared to ion channels. The SURFE²R N1 technology compensates for that with a large sensor size which allows for the measurement of up to 109 transporters at the same time to yield the best signal to noise ratio.
Any kind of membrane preparation containing the protein of interest can be used for measurements, e.g. membrane vesicles after cell disruption or proteoliposomes after reconstitution of purified proteins.
The SURFE²R N1 allows for determination of parameters such as EC50 or IC50. Since the time resolution of solution exchange is state-of-the-art, not only slow transport can be measured, but also fast binding reactions can be assayed and rate constants can be determined.