Member since: 2019
Organization: Boston Children's Hospital
Application Area: Analyze proportion of glycolytic vs oxidative metabolism in primary cells
"Results are consistent and reproducible. The kit it easy to use with primary cells. It can be used in conjunction with the cell mito stress test (in glucose-free media) to assess access to internal glycogen stores. The customer service used to be better with our previous representative, however, as long as you are using the kit "on label" there is good support."
The XF Glycolytic Rate Assay provides real-time measurements of glycolysis, allowing to detect glycolysis modulation in minutes in live cells. Researchers use this important assay to advance their understanding of immune cell activation, cancer cell metabolism and cell proliferation. The assay reports multiple key parameters, including basal glycolysis (a physiological rate) and compensatory glycolysis. Compensatory glycolysis is the glycolytic rate that cells can achieve when mitochondrial respiration is inhibited, which is indicative of cellular ability to manage energy demand under stress.
This assay should be performed using XF pH-defined media. Please refer to assay user guide to select the correct media.