Seahorse XF Glycolytic Rate Assay Kit by Agilent Cell Analysis

4.3
/
5.0
  |  1 reviews
The XF Glycolytic Rate Assay provides real-time measurements of glycolysis, allowing to detect glycolysis modulation in minutes in live cells. 


Seahorse XF Glycolytic Rate Assay Kit by Agilent Cell Analysis product image
Seahorse XF Glycolytic Rate Assay Kit
Request Pricing

Receive your quote directly from the manufacturer.




Average Rating: 4.3
1 Scientist has reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: Boston Children's Hospital



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Share this Review on LinkedinShare this Review on TwitterShare this Review on Facebook
Very good.
Rating: 4.3

  • Application Area: Analyze proportion of glycolytic vs oxidative metabolism in primary cells

"Results are consistent and reproducible. The kit it easy to use with primary cells. It can be used in conjunction with the cell mito stress test (in glucose-free media) to assess access to internal glycogen stores. The customer service used to be better with our previous representative, however, as long as you are using the kit "on label" there is good support."

Review date: 26 Nov 2019 | Seahorse XF Glycolytic Rate Assay Kit

The XF Glycolytic Rate Assay provides real-time measurements of glycolysis, allowing to detect glycolysis modulation in minutes in live cells.  Researchers use this important assay to advance their understanding of immune cell activation, cancer cell metabolism and cell proliferation.  The assay reports multiple key parameters, including basal glycolysis (a physiological rate) and compensatory glycolysis.  Compensatory glycolysis is the glycolytic rate that cells can achieve when mitochondrial respiration is inhibited, which is indicative of cellular ability to manage energy demand under stress.

This assay should be performed using XF pH-defined media. Please refer to assay user guide to select the correct media.

Features:

 

  • Each kit contains sufficient materials for performing 6 full plate assays 
  • It calculates and subtracts mitochondrial-produced acidification for a precise measure of glycolytic rate
  • It detects metabolic switching (such as Warburg effect) by reporting relative activity of mitochondrial respiration and glycolysis pathways
  • It measures before and after effects of metabolic modulators on glycolytic rates within a single assay well
  • This assay is best performed with Seahorse XF DMEM medium, pH 7.4 (Cat No. 103575-100) or Seahorse XF RPMI medium, pH 7.4 (Cat No. 103576-100). For other assay media options, refer to XF buffer factor protocol quick reference guide
  • Data analysis tool, the Seahorse XF Glycolytic Rate Assay Report Generator, is provided to simplify and transform raw data into organized, shareable summary reports anyone can understand.
  • The assay is for use with an XFe96, XF96, or XFe24 Analyzer. not compatible with XF24 and XF24-3 analyzers. For more information, please see the Agilent Seahorse XF Glycolytic Rate Assay Kit product website