DNA-PK is required for the non-homologous end joining (NHEJ) pathway of DNA repair, which rejoins double-strand breaks. It is also required for V(D)J recombination, a process that utilizes NHEJ to promote immune system diversity.
Mature B and T cells are critical components for an adaptive immune system. DNA-PK knockout rats have severe combined immunodeficiency (SCID) and lack of both B and T cells, due to their V(D)J recombination defect.
Rats deficient in the Prkdc gene produce no mature B or T cells. This SCID rat is a useful model for cancer, xenografts, vaccine development, and autoimmune and infectious disease study.
Prkdc Knockout Rat Characteristics: