The Orbit 16 platform takes the pain out of bilayer painting and subsequent recordings as it enables fast and successful data generation thanks to the rapid automated formation of 16 bilayers at once and their subsequent fully parallel recording.
The Orbit 16 is equipped with a dedicated low-noise 16 channel amplifier (Elements s.r.l.) by default enabling the convenient fully parallel recording of 16 bilayers at highest bandwidths. Furthermore, most commercially available amplifiers are compatible with the Orbit 16, including the 16-channel Triton amplifier from Tecella. For single channel amplifiers like the EPC-10 or Axopatch, the Orbit has a built-in multiplexer switchboard, allowing for recordings from the individual bilayers by automatically switching through the recording sites.
The recording module of the Orbit 16 is supporting Ionera's MECA 16 recording chips: Lipid bilayers are formed on an array of 4 x 4 circular microcavities in highly inert polymer. Each microcavity accomodates an individual integrated Ag/AgCl-electrode. The bilayers can be easily and repeatedly zapped and re-formed in an automated fashion. After bilayer formation ion channels or nanopores are reconstituted via self-insertion, proteoliposome fusion or dilution from detergent micelles.