Optima XE Ultracentrifuge

This application note provides a comprehensive guide to continuous flow centrifugation, describing what equipment might be needed to perform this technique as well as tips and FAQs. Continuous flow centrifugation is a method that can be employed to drastically decrease operational times for sample processing particularly if the sample size is large, sedimentation rate is low or the acceleration/deceleration rate is slow.

This application note provides insight into the inherent shortcomings that are present in traditional cellular toxicity assays, and how these shortcomings are magnified when the toxicity assay involves nanomaterial. Nanomedicine is a field common to materials scientists, chemists, biomedical engineers, biologists and medical scientists. Beckman Coulter tools enable quantification of cellular toxicity caused by nanoparticles; reducing time to remove aggregated particles and quantifying cell toxicity in the presence of single-walled carbon nanotubes.

This application note describes the characterization of a heteroassociating system. The thermodissociation is studied using band sedimentation analysis, whilst the electrostatic dissociation is studied using boundary sedimentation analysis. Analytical ultracentrifugation represents a powerful methodology for expressing HIV-1 viral components. In conjunction with appropriate data analysis methodologies, analytical ultracentrifugation can be used to examine sample purity, detect and characterize conformational changes, determine subunit stoichiometries, characterize assembly and disassembly mechanisms of macromolecular complexes, and measure equilibrium constants and thermodynamic parameters of associating systems.

Exosomes are small microvesicles released by all cell types, and proven to be involved in cancer metastasis. Exosome characterization and analysis comprise a fast, evolving research area even though their biological function has yet to be completely elucidated. In cell culture, fetal bovine serum (FBS) is usually incorporated into media, despite FBS containing extremely high levels of innate bovine extracellular vesicles which complicate downstream analyses. This application note explains the process for “home-brewing” exosome-depleted FBS by ultracentrifugation and compares the cell viability and media depletion percentage from two separate cell lines after treatment with different media types.

Tumor and normal cells may contain different subsets of microRNA expression profiles which can be found in exosomes. Therefore, monitoring the exosomal miRNAs can be used for early cancer screening and detection. This application note describes how the Agencourt RNAdvance Cell v2 kit from Beckman can be used to extract miRNA for this reason.

This application note presents a simple workflow using automation, centrifugation and dynamic light scattering (DLS), to purify and analyze exosome samples. The Biomek 4000 laboratory automation workstation and the Optima MAX-XP ultracentrifuge provide a consistent, reproducible and high-throughput method for exosome analysis.

Floor-standing ultracentrifuges and high-performance centrifuges are vital to many bioproduction processes. Instruments are run in Good Manufacturing Practice (GMP) environments, and must support compliance with these regulations. This application note describes how innovative features built into Optima XPN and Avanti JXN Series centrifuges support GMP compliance while eliminating much of the administrative work of other systems.

This presentation introduces Beckman Coulter’s versatile Avanti JXN-26 high-performance centrifuge. Learn about features including intuitive touch screen display, on-board user management, and remote monitoring. Also discover the new JA-14.50 rotor, designed for maximum throughput and performance.

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