Member since: 2015
Organization: KU Leuven
Application Area: Biomedical imaging
"This is very nice microscope for biologists. It offers 3D imaging with astigmatism introduced with cylindrical lens, and automatic calibration system as well as focus tracking/stabilization. However, it is a "closed" system so it is hard to make any modifications or upgrades without Nikon support. For scientists who like "custom" solutions I would recommend a bare body or one of the Olympus systems that seems to be more accessible."
STORM differs from conventional fluorescence microscopy in that it does not observe all the fluorescently labeled molecules on the sample at the same time, but activates only a very low percentage at any one given time. Repeating this process and acquiring multiple frames in which the molecules are localized with nanometer accuracy results in a final Super-Resolution image.