Member since: 2021
Organization: Bursa Uludag University
Not as expected, it should be improved.
Application Area: Isolation of total RNAs from plasma and whole blood for microarray
"Although we completed all the steps recommended in the literature and protocol, we could not isolate RNA at the expected concentration and quality. For high-throughput technologies, OD values are definitely very bad and not recommended."
Member since: 2011
Organization: UNIVERSITY OF HAWAI'I CANCER CENTER
I use QIAGEN for small RNA extraction from plasma forever.
Application Area:plasma RNA extraction for small RNA-seq and qPCR
"Well organized kit. I tried several kits and this one had the best RNA recovery rate from 200ul plasma, which is sufficient for QIAseq small RNA-seq library. Working time is also acceptable (12 samples/3h)."
The miRNeasy Serum/Plasma Kit is designed for purification of cell-free total RNA — primarily miRNA and other small RNA — from small volumes of serum and plasma. RNA from serum and plasma typically consists of molecules <100 nucleotides.
Purification can be automated on the QIAcube Connect.>