KRIBIOLISA™ Pembrolizumab (KEYTRUDA) ELISA by KRISHGEN BioSystems

Principle:

The method employs the quantitative enzyme immunoassay technique. Standards or samples are pipetted into microwells pre-coated with Anti- Pembrolizumab antibody and Pembrolizumab present in the sample and standards are bound by Anti- Pembrolizumab antibody. In the second step, Detection antibody is added and incubated. In the third step, an HRP conjugate is pipetted and incubated. Free HRP conjugate will be removed by washing. Addition of TMB substrate will develop blue color and intensity of blue color in wells is proportional to the concentration of Pembrolizumab present in standard or sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

• Calibrator Range: 0 ng/ml - 640 ng/ml
• Sensitivity: 8.25 ng/ml
• Sample Type: serum and plasma
• Shipping Condition: 2 - 8 Deg C
• Detection Method: Colorimetric
• Usage: For Research Use Only
• Research Area: Immune Checkpoints Therapeutic Drug Monitoring Cancer

Krishgen kits have over 1000 citations, with our ELISA kits being used for publications in 15+ countries.