Status:
ReviewerMember since: 2018
Organization: SUNY Downstate Medical Center
Great results, my number one option for ELISA assays!
Application Area: Analysis of human colon cancer cell's lines in response to inflammation
"This product is very effective and easy to use. The instructions are easy to follow, the sensitivity is in pg/ml (very low concentrations are detected) and are more affordable than other vendors."
Status:
ReviewerMember since: 2017
Organization: SUNY Downstate Medical Center
Great results! I will use it again
Application Area:Measurement of cytokine secretion in colon cancer cells
"This ELISA IL-8 is great! It gave good results and they matched what I expected to see in my colon cancer cells. The tech support was easy to talk to and very helpful. This ELISA is easy to use and the protocol steps are very straight forward. In addition, the price is adequate and better than the competitors. I will definitely buy more of these ELISA kits!"
Antigen Information
Accession Number: P10145
Gene ID: 3576
Gene Symbols: CXCL8; IL8
Protein Name & Synonyms: Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor) [Cleaved into: MDNCF-a (GCP/IL-8 protein IV) (IL8/NAP1 form I) Interleukin-8 ((Ala-IL-8)77) (GCP/IL-8 protein II) (IL-8(1-77)) (IL8/NAP1 form II) (MDNCF-b) IL-8(5-77) IL-8(6-77) ((Ser-IL-8)72) (GCP/IL-8 protein I) (IL8/NAP1 form III) (Lymphocyte-derived neutrophil-activating factor) (LYNAP) (MDNCF-c) (Neutrophil-activating factor) (NAF) IL-8(7-77) (GCP/IL-8 protein V) (IL8/NAP1 form IV) IL-8(8-77) (GCP/IL-8 protein VI) (IL8/NAP1 form V) IL-8(9-77) (GCP/IL-8 protein III) (IL8/NAP1 form VI)]
Target Species: Human
Assay Format
Detection Range: 1 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma): 2 fold
Sensitivity: 1 pg/ml
Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected: 1
Species Detected: Human
Compatible Sample Types: Cell Culture Supernatants; Cell Lysates; Plasma; Serum; Tissue Lysates
Design Principle: Sandwich-based
Method of Detection: Colorimetric
Quantitative/Semi-Quantitative: Quantitative
Solid Support: 96-well Microplate
Product Specifications
Size: 1, 2, or 5 x 96-Well Strip Microplate Kit
Product Features
Additional Notes
Application Notes
Kit Components
Other Materials Required
Protocol Outline
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.