Human IL-8 ELISA
RayBio® Human IL-8 (CXCL8) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Standard Curve
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Great results, my number one option for ELISA assays!
Analysis of human colon cancer cell's lines in response to inflammation
This product is very effective and easy to use. The instructions are easy to follow, the sensitivity is in pg/ml (very low concentrations are detected) and are more affordable than other vendors.
Review Date: 22 Jun 2018 | RayBiotech Inc.
Great results! I will use it again
Measurement of cytokine secretion in colon cancer cells
This ELISA IL-8 is great! It gave good results and they matched what I expected to see in my colon cancer cells. The tech support was easy to talk to and very helpful. This ELISA is easy to use and the protocol steps are very straight forward. In addition, the price is adequate and better than the competitors. I will definitely buy more of these ELISA kits!
Review Date: 19 Jul 2017 | RayBiotech Inc.
Assay Format
Detection Range
1 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Sensitivity
1 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum
Design Principle
Sandwich-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Quantitative
Solid Support
96-well Microplate
Protein Information
Accession Number
P10145
Gene ID
3576
Gene Symbols
CXCL8, IL8
Protein Name & Synonyms
Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor) [Cleaved into: MDNCF-a (GCP/IL-8 protein IV) (IL8/NAP1 form I) Interleukin-8 ((Ala-IL-8)77) (GCP/IL-8 protein II) (IL-8(1-77)) (IL8/NAP1 form II) (MDNCF-b) IL-8(5-77) IL-8(6-77) ((Ser-IL-8)72) (GCP/IL-8 protein I) (IL8/NAP1 form III) (Lymphocyte-derived neutrophil-activating factor) (LYNAP) (MDNCF-c) (Neutrophil-activating factor) (NAF) IL-8(7-77) (GCP/IL-8 protein V) (IL8/NAP1 form IV) IL-8(8-77) (GCP/IL-8 protein VI) (IL8/NAP1 form V) IL-8(9-77) (GCP/IL-8 protein III) (IL8/NAP1 form VI)]
Target Species
Human
Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
