Human IL-8 ELISA by RayBiotech Inc.

Manufacturer RayBiotech Inc.  |   Model: ELH-IL8
4.7
/
5.0
  |  2 reviews
RayBio® Human IL-8 (CXCL8) ELISA Kit for cell culture supernatants, plasma, and serum samples.


Human IL-8 ELISA by RayBiotech Inc. product image
Human IL-8 ELISA
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Average Rating: 4.7
2 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2018

  • Organization: SUNY Downstate Medical Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results, my number one option for ELISA assays!
Rating: 5.0

  • Application Area: Analysis of human colon cancer cell's lines in response to inflammation

"This product is very effective and easy to use. The instructions are easy to follow, the sensitivity is in pg/ml (very low concentrations are detected) and are more affordable than other vendors."

Review date: 22 Jun 2018 | Human IL-8 ELISA
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: SUNY Downstate Medical Center



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Great results! I will use it again
Rating: 4.3

  • Application Area:Measurement of cytokine secretion in colon cancer cells

"This ELISA IL-8 is great! It gave good results and they matched what I expected to see in my colon cancer cells. The tech support was easy to talk to and very helpful. This ELISA is easy to use and the protocol steps are very straight forward. In addition, the price is adequate and better than the competitors. I will definitely buy more of these ELISA kits!"

Review date: 19 Jul 2017 | Human IL-8 ELISA

Product Features

Sensitivity
1 pg/ml

Looking for higher sensitivity? Check out our new Ultrasensitive Biomarker Testing Service powered by SIMOA™ technology.

Detection Range
1 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor)
Accession Number
P10145
Gene Names
CXCL8, IL8
Gene ID
3576

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.