Member since: 2019
Organization: Earlham Institute
This instrument has single handedly improved the quality of DNA extractions.
Application Area: Purify DNA samples and remove smaller molecules
"It has helped us recover a number of samples where sample collection has been compromised resulting it poor quality DNA. Yields are really encouraging and resultant sequencing outputs well beyond our expectations."
The High Pass Plus™ gel cassette is part of the Pippin Family. As the name suggests, it is dedicated to our BluePippin “High-Pass” DNA size selection which has been a go-to method for increasing the read lengths for long-read sequencing.
High Pass size selection removes smaller DNA fragments from a sheared genomic DNA (or sequencing library) while collecting the remaining larger fragments above a tightly controlled size threshold. This way, larger molecules can be presented to the detector or droplet, and better sequencing performance can be achieved.
Long-read sequencing sample prep has been improving overall, in terms of set-up time and workflow. We decided to look at the High Pass as well to see if we could optimize the approach. To this end, we designed an entirely new gel cassette dedicated to High Pass– the High Pass Plus. We’re happy to say that we were able to cut the runtime in half and improve performance and yield.
The High Pass Plus cassette has a stocky separation column, so DNA has a shorter distance to travel. The wider column and increased taper provide higher resolution for a cleaner and more accurate size cut-off. The larger sample wells now allow a maximum load of 10ug (100% up from standard Blue Pippin cassettes). We’ve also increased the size of the elution module and surface area of the filtration membrane, bringing about improved sample recovery and reproducibility.