eaZYX is a high-end and yet easy-to-use software platform for image acquisition, analysis that is composed of three parts: eaZYX-IMAGING, eaZYX-PROOFING and eaZYX-AUTOMATION.
eaZYX-IMAGING is the image analysis core software engine
Unlike many image analysis softwares, eaZYX uses a holistic principle to ‘look’ at images. It does not segment pixel information to pull forward certain features in the image using a ‘pixel-by-pixel’ image segmentation. It applies the principles of ‘Scale Space’ theory to microscopic images (Van Osta et al., 2002). eaZYX uses the entire image content: the pixel features as well as their spatial context to identify objects (groups of pixels) based on shape, spectral and intensity characteristics.
These powerful tools were applied to create the patented object-based auto-focusing tools of the MIAS-2 readers (Geusebroek, 2000) that allow robust real-time auto focusing in extreme low light conditions. They also form the basis from which numerous eaZYX-IMAGING applications have been developed.
eaZYX-software supports high-end image analysis in two ways
eaZYX-IMAGING is your work environment to develop and store your own image segmentation scripts and applications. Within eaZYX-IMAGING, you will select (batches of) images from disk or server; you will select the image analysis application and initiate the analysis of the image (batch). eaZYX-IMAGING will produce detailed result data tables with descriptive statistics summaries for the identified objects. The result data files (and derived images) are ‘database ready’ for further analysis in spreadsheet programs, in bio-statistical software, in bio-informatics-, in drug discovery- or image databases for further evaluation.
eaZYX-PROOFING is a set of easy-to-use, on-image proofing tools for the researcher to review or approve large sets of assay results quickly. These proofing tools conveniently relate the output data tables directly to objects in images and vice-versa.