CRISPR Human Genome 80K Knockout Library (plasmid)
Cellecta’s 80K Single-module Human Genome-Wide CRISPR Library was designed to be small enough (~80,000 constructs) to enable convenient genome-wide “dropout viability” screens with most standard human cell modules. With 4 sgRNAs targeting each gene, all of which have been designed using the latest criteria for effective guides described by Doench, et al. and containing the Cellecta-designed HEAT sgRNA structure which we hav…
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High titer makes consistency and penetration of difficult cell models better.
Functional Genomics - lentiviral arrayed CRISPR libraries for target discovery
Because of high titer, result consistency and reagent delivery it is great. Particularly for physiologically relevant cell models which we are trying to enable for more target identification.
Review Date: 28 Jan 2020 | Cellecta
Cellecta’s 80K Single-module Human Genome-Wide CRISPR Library was designed to be small enough (~80,000 constructs) to enable convenient genome-wide “dropout viability” screens with most standard human cell modules.
With 4 sgRNAs targeting each gene, all of which have been designed using the latest criteria for effective guides described by Doench, et al. and containing the Cellecta-designed HEAT sgRNA structure which we have proven to generate more robust screening results than the standard sgRNA design, the library has deep enough gene coverage to generate efficient results.
Genome-Wide Coverage with a Convenient Single 80K Module
- Single library with ~80,000 constructs targeting approximately 19,000 protein-coding genes
- Each gene is targeted by 4 sgRNAs designed based on Doench, et al. criteria
- Targets all human protein-coding genes—over 19,000 gene targets.
The 80K Single-Module Human Genome-Wide CRISPR Library includes the following controls
- standard non-targeting controls
- non-specific control (sgRNA targeting introns throughout the genome)
- lethal sgRNA targeting generally essential genes (i.e., positive controls for dropout screens)
Brochures
Cellecta: Your Gene Knockout Solutions Partner
Discover Cellecta's CRISPR knockout portfolio in this brochure, including constructs, polled lentiviral libraries, screening service and customer cell lines.
CRISPR/Cas9 Genome-Wide gRNA Library for Target Identification
Genome-wide loss-of-function screening is a fundamental method to identify genes responsible for driving biological responses, and complex pooled lentiviral-based libraries expressing large numbers of genetic disruptors, such as shRNAs, make large-scale cell screening practical. This study investigates the potential differences in the RNAi and CRISPR HTGS technologies, by performing parallel dropout viability screens using module-1 human genome-wide CRISPR sgRNA library and module-1 human genome-wide RNAi shRNA library, both modules targeting the same set of 6,300 genes with the same number of effectors (sgRNA or shRNA) for each gene.
Screening for Genes Regulating TGF-β-Induced Apoptosis
TGF-β signaling has been extensively studied, and as such provided a good model system to assess the performance of Cellecta’s shRNA screening library. TGF-β, a multifunctional extracellular cytokine, inhibits cell proliferation by arresting cell cycle progression and inducing apoptosis; however, it can also promote metastasis and angiogenesis in some cancer cells. This acquired resistance to the growth-inhibitory function suggests disruption of the TGF-β apoptotic mechanism by genetic alternations that inactivate the signal transduction genes in the pathway.
Modified sgRNA Design Improves Results of CRISPR Knockout Screens
Optimization of the sgRNA design used in CRISPR knockout screens has been the goal of several research groups in recent years. This application note aims to address points raised in recent studies, including whether modifications of the 80 bases downstream of the target region can increase the rate or knock-out efficiency of the active CRISPR system. Showing that it is possible to increase the quality of sgRNA libraries, with just a few changes to the 3' region.
Optimizing CRISPR/Cas9 for Easy Knock Out, Activation or Repression of Genes
The CRISPR/Cas9 technology, easy to adapt and use, has become the latest gene editing go-to for life scientists. From the design of guide RNA to the expression of different forms of Cas9, Dr. Sylvain Baron, research scientist at Cellecta, shares his projects in optimizing several aspects of CRISPR to make it much easier to knock out, activate or repress genes.
Combining Gene Editing with Functional Analysis to Elucidate Disease Pathways
At AACR 2017, Paul Diehl, Ph.D., Chief Operating Officer, Cellecta, discusses how the company makes use of new technologies such as gene editing and gene synthesis in combination with next-generation sequencing to develop assays enabling researchers to investigate how gene function and gene expression are linked to tumorigenesis. He describes two approaches – loss-of-function screens and targeted RNA expression analysis – that Cellecta has developed to help investigators better understand that relationship.
