Member since: 2011
Organization: Molecular Devices Inc
Grow 3D structures without much assay development or special equipment.
Application Area: Culturing cancer spheroids
"It's easy to grow many cancer cell lines into 3D spheroids in these 384 well plates for performing toxicity studies. I've been successful with HCT116, DU145, HepG2, U2OS, and MCF7 cells forming either tight or loose 3D spheroids depending on the cell type. After simply allowing the spheroids to grow for 3-7 days, they can be treated, stained, and assayed directly in the round-bottom plate even if imaging on an automated high-content system (I used ImageXpress Micro). It took a short development time and did not require figuring out how to use an out of the ordinary labware consumable."