Diabetes is a public health problem. According to the WHO, the number of people with diabetes increased from 108 million in 1980 to 422 million in 2014.
Plasma glucose levels are essential for the evaluation of diabetes mellitus as well as gestational diabetes.
The principally used analytical methods are enzymatic assays, such as the hexokinase reaction using an enzyme involved in the metabolic pathway of glycolysis. These methods are recommended and highly standardized with an inter-laboratory imprecision (CV) < 2.6% (1.4.5).
The additive NaF - Sodium Fluoride (additive present in the glucose tubes, grey cap) on glycolysis becomes effective just 2 to 3 hours after blood sampling.
An in vitro decrease in plasma glucose of 5-7% per hour has been reported on non-centrifuged samples immediately after collection. Cellular activity continues to affect glucose concentration.
It’s known that the time from collection until separation of plasma and cells, temperature as well as cell count strongly affect glucose levels, possibly leading to false low results and subsequently to clinical misdiagnosis and incorrect patient therapy followed by increased health costs.
Having a sample tube with an anticoagulant that immediately preserves the glucose concentration is therefore essential for correct classifications.
Furthermore, it is interesting to have a sampling tube that blocks glycolysis at T0 thanks to an additive that inhibits the first enzyme (hexokinase) necessary for the first biochemical reaction of glucose degradation: Citrate.
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