Dymension 2D Gel Analysis Software used at Specialist Institute To Identify Proteins Induced by an Anti-Addictive Compound
21 Feb 2008Syngene, a world-leading manufacturer of image analysis solutions, today announced, Dymension, its powerful software for the analysis of 2D protein gels is being used at the Open Mind Institute (OMI) in Slovenia, a new centre for studying the effects of medicinal plants, to determine the effects the anti-addictive alkaloid, ibogaine have on the brain.
Researchers in the OMI in collaboration with scientists at the University of Ljubljana in Slovenia are using Dymension to accurately determine any post ibogaine treatment differences in silver stained rat brain proteins run on 2D gels. The protein profiles are used to establish which proteins are up or down-regulated. This research could lead to a better understanding of the pharmacodynamics of anti-addiction therapies.
Dr Roman Paškulin, Director of the OMI explained: “We are relatively new to proteomic analysis so we chose to install Dymension software because we want to rapidly perform complicated analysis, and of the software packages we reviewed, this was visually easier to navigate and simpler for everyone to use.”
Dr Paškulin added: “Using Dymension to analyse our 2D gel images in association with MALDI-TOF on the resulting protein spots, we have seen increases in four metabolic enzymes after ibogaine treatment and are now looking at the significance of these findings.”
Laura Sullivan, Syngene’s Divisional Manager said: “We are delighted to see our Dymension software being used to accelerate the pace of such interesting research. The software has enabled scientists at the OMI and the University of Ljubljana to analyse their 2D gel results so rapidly they have already published a paper and is a testament to how quick and simple it is to integrate and work with Dymension software in any proteomics project.”
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DYMENSION 1
SyngeneRevolutionary 2DGE Software Analysis of single 2D gels - Dymension 1 is ideal for users looking for software to analyse spots between samples containing one 2D gel only. It offers a range of automatic image correction features and methods for resolving and analysing spots on single gels. The software can be easily upgraded to Dymension 2 or 3 if the proteomics needs of the laboratory change.
DYMENSION 2
SyngeneRapid Analysis of Multiple 2D Gel Sets DYMENSION 2 has all the features of DYMENSION 1 but has the added ability to detect and compare protein spot maps in multiple gel sets. The software offers many background correction and noise filtering features including a SYPRO® Ruby filter, which can detect and automatically remove spots caused by SYPRO® Ruby crystals adhering to the gel, thus saving users valuable time editing their image. DYMENSION 2 is suitable for users that need to determine the amount of protein present before and after drug or protein induction treatments. The software automatically detects and assigns statistical confidence to each and every difference in spot normalised volume, thus accurately highlighting proteins of specific interest. The results of the analysis are displayed as a dynamically linked table, 3D spot profile, bar chart and scatter plot, which make it easy to compare many expression profiles simultaneously and therefore accurately pick proteins suitable for further analysis.
DYMENSION 3
SyngeneQuick and Easy Analysis of all 2D Gel Applications The exciting DYMENSION 3 software has all the functionality of DYMENSION 2, so can be used to analyse multiple gel sets. In addition, DYMENSION 3 is capable of analysing multi-stained fluorescent gel images stained with up to three contrasting fluorescent labels, for example, Cy™2, Cy™3 and Cy™5. This feature allows users to analyse multiple sets of any type of 2D protein gel, regardless of how it was stained, using only one software package.