Contamination Free High Throughput Preparation of Media Filled Petri Dishes

7 Jun 2015
Kathryn Rix
Administrator / Office Personnel

The Institut Curie, Paris, France, is a world leader in oncology research. Research work at the Institut Curie requires petri dishes for a wide range of applications including growing bacteria, yeasts and moulds, making it necessary to regularly produce 10 to 12 different types of media in varying quantities. The assurance of reliable, high throughput production of these petri dishes which are free of any contamination is critical to ongoing research work.

The Institut Curie has reported how it has standardised using MEDIACLAVE media preparation and MEDIAJET automated petri dish filling systems from INTEGRA Biosciences to prepare its petri dishes.

Fatima Dekmous, Departmental Head of the Wash facility at Institut Curie commented “I have been preparing media in Petri dishes for over 13 years, and have the pleasure of working closely with the researchers based here at the Institut. The research teams are very well organized and give two to three days' notice of their Petri dish needs. To satisfy their needs, it is very important to be able to rely on the quality of the equipment used. We have been using INTEGRA devices for more than 15 years and most recently acquired a MEDIAJET and MEDIACLAVE with printer. This equipment is a great help in our work in terms of user-friendliness, speed and, above all, reliability. It is compact and easy to use guiding us clearly through handling and diagnostics. Maintenance is also very simple. For us, the printer has proved essential. We process 800 to 1000 dishes a week and, while this saves us time, it is also important to limit handling of the dishes. There has never been any contamination”.

She added “We use vented Petri dishes, which work perfectly with the MEDIAJET automated petri dish filling system. I should also say that, as well as trusting the equipment, I have full confidence in the after-sales service provided by INTEGRA. When you need them, INTEGRA have always been very responsive. I recommend MEDIAJET and MEDIACLAVE for high throughput preparation of top quality media filled petri dishes without hesitation”.

MEDIAJET - Petri Dish Filler

INTEGRA Biosciences

MEDIAJET – Reliable walk-away media dispensing system The MEDIAJET media filling system offers the unique flexibility to fill Petri dishes as well as test tubes of various sizes. The compact MEDIAJET provides truly reliable, walk-away operation. The dish guidance system is monitored throughout the filling process. Operational downtime due to 'dish jams' resulting from variations of the Petri dishes is completely eliminated. The MEDIAJET includes a shaker mode for facilitating pour plate applications.It is the perfect complementation to the INTEGRA MEDIACLAVE media preparator range as it allows the continuous filling of up to thirty litres media into Petri dishes. The TUBEFILLER option is the perfect expansion to the functionality of MEDIAJET, as it allows the conversion into a test tube filler in just a minute for the continuous processing of various test tubes. Features and benefits: • Controlled dish guidance for reliable walk-away operation • Fast Petri dish processing for filling up to 900 plates per hour. • Shaker mode for pour plate applications.• TUBEFILLER option for the filling of up to 1000 test tubes in one hour.

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MEDIACLAVE 10 - Media sterilizer

INTEGRA Biosciences

MEDIACLAVE 10 – For fast, reliable and safe media preparation The MEDIACLAVE 10 allows the rapid and gentle sterilisation of 1-10 L culture medium. Precise control of process parameters during the sterilisation process guarantees media of constant high quality. The intuitive multilingual user interface and the simple programming make it very easy, even for untrained users, to operate the MEDIACLAVE 10. Media preparation by the MEDIACLAVE 10 is very easy. The culture medium can be dissolved directly within the instrument. Automatic self checks and several monitoring systems guarantee highest operational safety. The MEDIACLAVE 10 can be conveniently connected to the INTEGRA Biosciences MEDIAJET, allowing the automated dispensing of media into up to 540 Petri dishes at the touch of a button. Features and benefits: • Flow heater system for fast and gentle media preparation. • Powerful bidirectional stirrer to guarantee homogeneous media. • Controlled vessel deaeration for complete media sterilisation • Indication of service intervals to avoid unscheduled downtime. • TÜV certified for compliance to international safety standards. • Integrated web server for online documentation of the process. • Electronically signed log files according to FDA and EU guidelines.

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