New Instrument from Thermo Scientific Offers Fast Transfer of Proteins for Western Blot Detection
6 Apr 2014
A new instrument is now available that offers fast transfer of proteins from polyacrylamide gels to typical Western blot membranes, for subsequent antibody detection, speeding up Western blot methods. Thermo Fisher Scientific showcased these solutions during analytica 2014.
The Thermo Scientific Pierce G2 Fast Blotter provides researchers a tool to perform rapid semi-dry transfer of 10-350kDa proteins from polyacrylamide gels to nitrocellulose or PVDF membranes, for subsequent Western blot detection. When used with the Thermo Scientific Pierce1-Step Transfer Buffer, the Pierce G2 Fast Blotter transfers proteins in 5-10 minutes without the need for gel pre-equilibration. This significant reduction in protein transfer time is accomplished by optimizing the ionic strength of the transfer buffer and increasing the current [amps (A)/cm2] flowing through the transfer stack.
The Pierce G2 Blotter has an easy-to-use color LCD touchscreen with pre-programmed transfer methods that allow users to quickly create, run and save custom transfer methods. The system has been verified to work with commonly used pre-cast and homemade SDS-PAGE gels and can be used to simultaneously transfer up to four mini-sized gels or two midi-sized gels.
The Pierce G2 Blotter is the most recent addition to the Thermo Scientific line of Western blot instruments and reagents, which includes the Thermo Scientific MYECL Imager for Western blot capture and analysis and the popular Thermo Scientific SuperSignal Substrates.
Related Products
Request Quote for All Products
Thermo Scientific Pierce G2 Fast Blotter
Thermo Fisher ScientificThe Thermo Scientific Pierce G2 Fast Blotter is designed specifically for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in 5 to 10 minutes. The Pierce G2 Fast Blotter features an integrated power supply optimized to deliver consistent, high-efficiency protein transfer with commonly used pre-cast and homemade gels (SDS-PAGE) and nitrocellulose or PVDF membranes. The large cassette provides for simultaneous transfer of up to four mini-sized gels or two midi-sized gels. The blotter has an easy-to-use color LCD touchscreen interface and pre-programmed transfer methods for different numbers and sizes of gels and molecular weight ranges of proteins. The easy-touch programming feature allows custom transfer settings to be quickly created, saved and run. Features: Fast – transfer proteins in 5 to 10 minutes when used with 1-Step Transfer Buffer Efficient – achieve high transfer efficiency with a broad range of protein sizes (10-300kDa) compared to conventional semi-dry or wet (tank) transfer methods Integrated power supply – seamless operation between control unit and cassette provides consistent high efficiency protein transfer Easy-touch programming – access pre-programmed transfer methods or create, save and run customized transfer methods Convenient – simultaneously transfer 1 to 4 mini gels or 1 to 2 medium-sized gels Versatile – use with Pierce 1-Step Transfer Buffer for rapid blotting programs or Towbin transfer buffer for conventional semi-dry transfer methods