Product News: AnaSpec Launches Industry’s First Fluorimetric Assay for Measuring Glutaminyl Cyclase ActivityAnaSpec is pleased to announce the release of the first commercially available 96-well format, fluorimetric assay for measuring Glutaminyl Cyclase (QC) activity - the SensoLyte® Green Glutaminyl Cyclase Activity Assay Kit.
Glutaminyl cyclase (QC), an enzyme found in animals and plants, catalyzes the cyclization of N-terminal Glutamine, and to a lesser extent Glutamate, to pyroglutamate. This post-translational modification (PTM) to pyroglutamate, present in many biologically active peptides and proteins, protects against degradation by aminopeptidases and in many cases determines functionality of protein or peptide.[2-7]
Pyroglutamate modification sometimes may lead to negative side effects. pGlu (or pE) modified β-amyloid peptides (pGlu-Aβ) aggregate much faster than non-pyroglutamic Aβ and form toxic oligomers that may damage neurons. The aggregation of β-amyloid plaques is one of the hallmarks of Alzheimer’s Disease (AD).[8-10] CCL2 (monocyte chemoattractant protein 1, MCP-1), implicated in a number of diseases has in its mature form an N-terminal pGlu. This modification protects it against aminopeptidase degradation, and improves receptor activation and signal transduction in-vitro
. Increased levels of pE-CCL2 has been implicated in thyroid carcinoma proliferation and metastasis, and atherosclerosis, and other inflammatory disorders. Thus, Glutaminyl Cyclase (QC) inhibitors are attractive drug candidates. References
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