NMR spectroscopy can provide unique contributions for the description of cellular processes, as it is suitable not only for characterizing the structural and dynamical properties of biomolecules but also for describing transient interactions and functional events with atomic resolution possibly in a cellular context. This requires the development of suitable methodologies capable of addressing multiple, specific, and sometimes non-conventional aspects for describing functional processes in cells.
In this on-demand SelectScience® webinar, Prof. Lucia Banci, University of Florence, describes how the power of NMR can be harnessed for describing cellular pathways at atomic resolution in a cellular environment. Banci presents examples for pathways responsible for cellular copper trafficking and for the biogenesis of iron-sulphur proteins.
Through an integrated approach, by increasing the complexity from single protein structures to protein complexes to the functional reaction steps, these processes are described in their cellular context within a molecular perspective. Furthermore, the exploitation of UHF NMR spectrometers, such as that operating at 1.2 GHz for in-cell NMR applications, is explored.
The webinar is introduced by Dr. Rainer Kuemmerle, Vice-President at Bruker Biospin and Head of NMR Applications, who talks briefly about the development of the world-first NMR system operating at 1.2 GHz magnetic field, which is installed at Florence University.
Read on for highlights from the live webinar’s Q&A session or register to watch the webinar on demand.
LB: 15N has a lower gamma than protons and is a much less affected of the paramagnetic center. It is a good strategy and we have developed this NMR experiment because nuclei with low gamma are much less affected by the paramagnetic center. If the paramagnetic effect is not too dramatic, we can exploit the TROSY effect. The paramagnetic effect depends quite a lot on the nature of the metal ion and its coordination. Clusters have a different electronic structure, the electron spin level and spin relaxation are different, so it really depends on the specific paramagnetic center. If the paramagnetic effect is still dramatic, the TROSY effect does not help much because the cross-correlation is overcome by the paramagnetic relaxation. If the paramagnetic effect is minor, we can exploit that. 15N is a very good nucleus to be exploited, while 19F has a gamma that is very high and is more affected by paramagnetic centers.
LB: Not particularly, except in a few cases where there are specific folds of metal binding, especially when proteins are very small and with a lot of ligands. They have an arrangement that determines the fold by the binding of the metal. We found folds that are widespread and present in many other types and classes of proteins and the protein acquired the ability to bind to the metal. In some proteins, they retain two functions, the specific fold and the ability to bind the metal. The fold is not the immediate way to recognize whether a protein is metal binding or not, it has more to do with the organization of the specific metal-binding site. The presence and number of potential ligands for metal ions can then indicate if it is a metal-binding protein or not.
LB: It is very small. We did a systematic analysis of the genomes with respect to the metal binding of transition metal ions and found we have 2% of iron-binding proteins. Iron can be either paramagnetic, or some of the oxidation states of iron are diamagnetic. We have very few manganese- and copper-binding proteins, but despite being very few they are present in resting states in the cell as CuI, which is diamagnetic. Only during reaction does it become CuII - that is paramagnetic. The fraction of paramagnetic metal binding proteins is very small, roughly around 2%.
LB: This has been exploited by many research groups. The magnetic anisotropy of the metal ions can be used to make a fraction of the orientation of the molecules align with the magnetic field and then carry out measurements. When you have partial orientation of the molecules, the paramagnetic center has magnetic anisotropy, and it depends on the nature of the specific metal ions and each coordination. Some ion-containing systems have magnetic anisotropy, like heme which contains an iron III low-spin system. Here in Florence, we have exploited lanthanides as tags to exploit the ability of molecules to self-orientate due to the magnetic anisotropy of the paramagnetic center.
Q: What cryoprobe do you have on the 1.2 GHz, do you use TCI and/or TXO?
LB: We have the TCI and I hope soon we get the TXO. Now, we collect data with proton detection, but we do want to apply carbon detection, and, direct detection of 15N. We have had very good results, so we look forward to collecting data on our samples.
Q: Does magnetic field strength affect the health of cells used in the in-cell NMR experiments?
LB: In in-cell NMR, we are working on proteins in the cytoplasm that are moving freely, because if they weakly interact with the membrane or with other very large components then their motion will slow down, the lines become broad and we do not detect them. This is magnetic field independent; we have tested it at 950 MHz and we have tested at 1.2 GHz and we see that, particularly for structured proteins, we have a huge improvement going to a higher field, in terms of sensitivity, resolution and TROSY effect. nitrogen-15 TROSY in a cell improves a lot because it is theoretically much better than at a lower field. However, there is no self-orientation, no problem in that respect. For larger proteins with intrinsic homogeneity in the sample, improvements will not be as large as in intrinsically disordered proteins which are much more mobile and so have sharper lines.
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