Validation of SpectraSlide AP-1 for reliable CHO-K1 and HEK293 cell counting and viability assays

26 Nov 2025

Mammalian cell lines such as CHO-K1 and HEK293 are essential for the production of therapeutic proteins, monoclonal antibodies, and viral vectors, and are widely utilized in biopharmaceutical research and development. These cell lines are preferred for their robust protein expression, human-compatible post-translational modifications, and suitability for both transient and stable expression systems.

In this application note, Logos Biosystems evaluates the performance of the SpectraSlide® AP-1 for viability and concentration analysis of CHO-K1 and HEK293 cells using the LUNA-FX7™, and compare its results to those obtained with the PhotonSlide™.

SpectraSlide® AP-1

Logos Biosystems

The SpectraSlide® AP-1, designed for the LUNA-FX7™ automated cell counter, is an all-in-one cell counting slide that simplifies the sample prep process - saving time & money. No pipettes and no stains needed! Simply load a sample directly on the slide, insert into the LUNA-FX7™ and press COUNT.

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LUNA-FX7™ Automated Cell Counter

Logos Biosystems

The LUNA-FX7™ is our most powerful cell counter to date, with unmatched cell counting accuracy, new 1, 3 and 8-chamber slide options, state of the art optics, dual fluorescent and brightfield detection, and precision autofocus. The LUNA-FX7™’s new features such as the Bioprocess mode, Quality Control mode, and the CountWire™ (21 CFR Part 11 compliant) software will help companies and facilities meet and surpass quality and regulatory requirements.

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