New Feature in 2D Protein Gel Analysis Software Improves Confidence and Productivity in Proteomics Research
11 Jun 2007Syngene, a world-leading manufacturer of image analysis solutions, today announced its Dymension software for automated 2D protein gel analysis now includes common spot outlines, a revolutionary algorithm for automatically detecting presence or absence of proteins in large data sets. This exciting feature saves time with proteomics workflow by generating data, with minimal manual post-editing which can be statistically analysed to confidently identify the correct proteins for costly downstream research.
The unique algorithm in Dymension that produces common spot outlines firstly warps all the gel images in an experiment together to create an averaged gel image and then performs detection on this image. These common spot outlines are overlaid onto each gel in the experiment’s series.
With Dymension's method of producing common spot outlines, each spot exists on every gel so results are less affected by noise and are therefore more representative of the common features of the underlying data. This means scientists can run less duplicate experiments, thus saving money on consumables and gel handling time.
Laura Sullivan, Syngene's Divisional Manager stated: "The resources required to analyse proteins identified from 2D gels can be considerable so scientists want to ensure they are working with the right proteins from the outset and this is where Dymension can help."
Laura Sullivan added: "We are pleased with this method of common spot outlines in Dymension because it contributes to gaining statistical confidence in 2D gel results from even large data sets. Using Common Spot Outlines, we are confident researchers can save time in up and downstream laboratory processing, which will ultimately increase the productivity of their proteomics research."
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DYMENSION 1
SyngeneRevolutionary 2DGE Software Analysis of single 2D gels - Dymension 1 is ideal for users looking for software to analyse spots between samples containing one 2D gel only. It offers a range of automatic image correction features and methods for resolving and analysing spots on single gels. The software can be easily upgraded to Dymension 2 or 3 if the proteomics needs of the laboratory change.
DYMENSION 2
SyngeneRapid Analysis of Multiple 2D Gel Sets DYMENSION 2 has all the features of DYMENSION 1 but has the added ability to detect and compare protein spot maps in multiple gel sets. The software offers many background correction and noise filtering features including a SYPRO® Ruby filter, which can detect and automatically remove spots caused by SYPRO® Ruby crystals adhering to the gel, thus saving users valuable time editing their image. DYMENSION 2 is suitable for users that need to determine the amount of protein present before and after drug or protein induction treatments. The software automatically detects and assigns statistical confidence to each and every difference in spot normalised volume, thus accurately highlighting proteins of specific interest. The results of the analysis are displayed as a dynamically linked table, 3D spot profile, bar chart and scatter plot, which make it easy to compare many expression profiles simultaneously and therefore accurately pick proteins suitable for further analysis.
DYMENSION 3
SyngeneQuick and Easy Analysis of all 2D Gel Applications The exciting DYMENSION 3 software has all the functionality of DYMENSION 2, so can be used to analyse multiple gel sets. In addition, DYMENSION 3 is capable of analysing multi-stained fluorescent gel images stained with up to three contrasting fluorescent labels, for example, Cy™2, Cy™3 and Cy™5. This feature allows users to analyse multiple sets of any type of 2D protein gel, regardless of how it was stained, using only one software package.