Modular DNA Assembly of PIK3CA Using Acoustic Liquid Transfer in Nanoliter Volumes
3 Dec 2018In this study, NEBuilder® HiFi was combined with the 25 nL increment dispensing Labcyte Echo® 525 Liquid Handler to assemble a modular oncogene construct encoding phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) over five modular pieces. The goal of this experiment was to utilize the Echo 525 Liquid Handler in assembling five, five-piece assemblies by substituting modular DNA pieces at the nanoliter scale. The Labcyte Echo Liquid Handler enables lower-cost methods and workflows to produce high-quality synthetic DNA constructs which expands design-based testing with higher throughput and affords the scientist a broader biological landscape to interrogate.
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Echo® 525 Liquid Handler
Beckman Coulter Life SciencesThe Echo 525 platform, like the other Echo liquid handler models, is designed to use sound energy to transfer reagents. Unlike the other Echo models, the Echo 525 platform transfers at a larger volume increment (25 nL). This enables the more rapid transfer of aqueous reagents required in biochemical and genomics assays setup.