Application Note: Western Blot Normalization with Azure Biosystems
16 September 2015
Normalization is critical for obtaining accurate, quantitative data from Western blots. Normalization accounts for unequal loading of samples across the lanes on a gel, and for differences in transfer efficiency across a blot. Traditionally, protein normalization for Western blots involves comparing the relative abundance of a protein of interest to that of an unrelated protein, the normalization or loading control. This application note explains a total protein normalization (TPN) method, in which the relative abundance of the protein of interest is compared to the total protein content of the sample.