Application Note: Vitamin D Level Determination in Serum
16 February 2016

Vitamin D3 (cholecalciferol) is essential for the human body. Low levels of vitamin D3 increase the risk of illness from a range of disorders. The serum level of vitamin D is monitored to exclude deficiency symptoms. As vitamin D products and nutritional supplements often contain vitamin D3 and/or vitamin D2 (ergocalciferol), usually the concentration of 25-hydroxy vitamin D3 (25-OH-D3, calcidiol) is determined. As vitamin D2, D3 and 25-OH-vitamin D3 are structurally similar substances, the (U)HPLC separation is therefore very challenging. Often, special columns in combination with complex eluent mixtures have to be used. This application note demonstrates the determination of vitamin D using YMC-Triart C18 ExRS.