Visualize gene expression and splice junctions at the single-cell level using the RNAscope and BaseScope in situ hybridization assays

16 Mar 2020

This application note highlights how the BaseScope ISH assay allows for the visualization of splice junctions between adjacent exons and/or retained introns in order to characterize alternative splicing events in cells and tissues.

BaseScope™ Assays

Advanced Cell Diagnostics

The BaseScope™ Assay is a unique product from ACD, based on the same platform of proven and established RNAscope™ technology that has been cited in over 2300 publications. This powerful assay provides new data dimensions and unique insight into biological mechanisms. The BaseScope™ Assay enables applications such as the detection of exon junctions/splice variants, short/highly homologous RNA sequences (50-300 bases), and point mutations at single cell sensitivity.

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Visualize gene expression and splice junctions at the single-cell level using the RNAscope and BaseScope in situ hybridization assays