Utilizing a Hybrid Mass Spectrometer to Enable Fundamental Protein Characterization: Intact Mass Analysis and Top-Down Fragmentation with the LTQ Orbitrap MS

Thermo Scientific LTQ Orbitrap XL - The Most Confident Protein Identification The Thermo Scientific LTQ Orbitrap XL hybrid FTMS (Fourier Transform Mass Spectrometer) supports a wide range of applications from routine compound identification to the most challenging analysis of low level components in complex mixtures. The LTQ Orbitrap XL is based on the fast and highly sensitive Thermo Scientific LTQ XL linear ion trap and the patented Orbitrap* technology. The LTQ Orbitrap XL features a new HCD collision cell for ultimate flexibility in fragmentation experiments for advanced proteomics and small molecule research. Key features of the Thermo Orbitrap XL include: Outstanding Mass Accuracy, Resolution, Dynamic Range and Sensitivity • Featuring the new HCD collision cell for ultimate flexibility in fragmentation experiments for advanced proteomics and small molecule research • Superior mass accuracy for lower false positive rates (FPRs) • Parallel acquisition mode for optimized productivity From Protein ID to Biomarker Discovery with the Thermo LTQ Orbitrap• Excellent sensitivity and fragmentation efficiency • Reliable identification and quantitation in one experiment High quality data from the LTQ Orbitrap XL for reliable de novo sequencing • Superior mass accuracy and high resolution leading to confident de novo sequencing results • Multiple collision modes (CID and HCD) for comprehensive structural information

The Thermo Scientific LTQ Orbitrap XL ETD is the most powerful instrument for proteomics analyses: It combines three different and complementary fragmentation techniques CID, HCD, and ETD with the proven benefits of Orbitrap technology. It is the most comprehensive solution for complex PTM analysis, intelligent sequencing of peptides, top-down and middle-down analysis, and protein quantitation via stable isotope labelling such as iTRAQ or label-free quantitation.

Building on a strong tradition of innovation, the Thermo Scientific LTQ Velos provides significantly enhanced performance for complex sample analyses. The revolutionary dual-pressure ion trap and S-Lens ion optics combine to deliver increased ion transmission along with greater efficiency of ion trapping and fragmentation. The result is the fastest and most sensitive ion trap for virtually all applications. The LTQ Velos™ is the latest evolution in the award winning LTQ family of linear ion trap mass spectrometers. It offers significant improvements in data acquisition rate, sensitivity, and mass resolution. S-lens ion optics increase ion transmission and shorten trap fill time, improving instrument sensitivity and data acquisition rate More efficient dual-pressure ion trap simultaneously increases scan speed and mass resolution Predictive Automatic Gain Control reduce cycle times, yielding improved data acquisition rates Improved sensitivity and mass resolution increase confidence in identification of low-level analytes Increased performances enables simultaneous identification, structural elucidation, and quantification of complex samples Upgradeable to the ultra-high resolution and outstanding mass accuracy of OrbitrapTM technology