ResourceLife Sciences
True 3D analysis of an intracellular autophagic pathway in a 3D spheroid model
18 Nov 20193D culture models have been used for predicting drug efficacy or toxicity. In this application note, we focus on an intracellular autophagic pathway. First, we confirmed the usefulness of the EGFP-LC3 probe for monitoring the models’ autophagic status. Then, Olympus’ true 3D analysis workflow using the FLUOVIEW™ FV3000 confocal microscope and NoviSight™ software was used to quantitatively evaluate the effect of a drug candidate called chloroquine. We demonstrated that this analysis workflow offers an alternative to profiling drug candidates using in vivo studies.
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Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Software PlatformsSoftware platforms are useful for various stages of laboratory experiments from data collection to data storage and processing. For instance lab software is available for system control, data management, data analysis and qualification / validation.Fluorescence MicroscopyFluorescence microscopy has become an essential tool in biology, as well as in materials science. The application of many fluorochromes has made it possible to identify cells and sub-microscopic cellular components with a high degree of specificity. Using multiple fluorescence labels, different probes can simultaneously identify several target molecules.ConfocalMicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.SpheroidsSpheroids are clusters of cells that have been grown in 3D culture to be used as <i>in vitro</i> model systems. These 3D microtissues can be used for toxicology testing, DMPK studies and many other applications involving cell analysis.Drug DevelopmentDrug development refers to the process of bringing a new drug to market.