Application Note: Sub-Nanogram Level Intact Monoclonal Antibody Quantitation by LC/Q-TOF
27 January 2018

Quantifying biopharmaceuticals by studying the intact protein levels avoids limitations seen in the more common targeted multiple reaction monitoring techniques, but can face problems with analytical sensitivity and reproducibility. Using the latest developments in chromatography and the high performance Agilent 6545XT AdvanceBio LC/Q-TOF, this study establishes a highly sensitive and reproducible quantitative analytical method that spans an impressive linear dynamic range.