Application Note: Stimulation of human peripheral blood mononuclear cells
6 June 2023
Human peripheral blood mononuclear cells (PBMCs) are routinely isolated from
blood samples and then used in several fields of research including autoimmune
disorders, infectious diseases, vaccine development and cancers. In this application note, learn more about the ELISpot
Assay, which monitors ex vivo cellular immune responses to antigenic stimuli. The Agilent BioTek Cytation 7 cell imaging multimode reader is used in conjunction with the Agilent BioTek Gen5 microplate reader and imager software to quantitate changes in
cytokine secretion in PBMCs using the colorimetric ELISpot assay format.