ResourceSpectroscopy

Spectral Flow Cytometry expanded to Visible and Near Infrared Fluorescence Spectroscopy

18 Sept 2014

The inherent design of a flow cytometer gives a unique advantage in multiparameter measurements on single particles, where each fluorphore is matched to a unique detector. However, the long standing goal of collecting complete emission spectra of spectrally labeled particles has remained elusive. In recent years Professor John Nolan and his group at the La Jolla Bioengineering Institute in San Diego have made significant strides toward this goal by coupling high-throughput dispersive spectrometers to standard design flow cytometers.

HoloSpec F/1.8 Imaging Spectrograph

Oxford Instruments Andor

  The new Andor Holospec is the ideal solution for collecting more light and achieving better signal-to-noise ratio faster, which is critical for applications such as micro-Raman mapping, microfluidics, real-time medical diagnosis (point-of-care analyzers) or stand-off bacteriologic agents or explosives detection.   The superb light collection efficiency capabilities of the superfast F/1.8 Andor Holospec spectrograph platform provides a perfect match to Andor’s ultra-sensitive CCD, EMCCD and ICCDs detectors, offering the most sensitive and versatile detection solution on the market for Visible or Near-Infrared spectroscopy. The Andor Holospec is the ideal solution for collecting more light and achieving better signal-to-noise ratio faster, which is critical for applications such as micro-Raman mapping, microfluidics, real-time medical diagnosis (point-of-care analyzers) or stand-off bacteriologic agents or explosives detection. The Holospec also offers aberration-corrected optics for excellent multi-track capabilities, enabling high density fibre optics simultaneous acquisitions with extremely low crosstalk, even on narrow spectroscopy sensors. Its rugged and compact design makes it an ideal tool for challenging industrial or in-the-field applications, while still offering research-grade performance suitable for academic research.

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Spectral Flow Cytometry expanded to Visible and Near Infrared Fluorescence Spectroscopy