Spectral Flow Cytometry expanded to Visible and Near Infrared Fluorescence Spectroscopy

The inherent design of a flow cytometer gives a unique advantage in multiparameter measurements on single particles, where each fluorphore is matched to a unique detector. However, the long standing goal of collecting complete emission spectra of spectrally labeled particles has remained elusive. In recent years Professor John Nolan and his group at the La Jolla Bioengineering Institute in San Diego have made significant strides toward this goal by coupling high-throughput dispersive spectrometers to standard design flow cytometers.