Application Note: Site Saturation Variant Libraries
21 June 2019

Protein engineering screens using single site variant libraries allow researchers to explore a protein’s sequence space and investigate the relationship between sequence and protein structure and function. Many methods exist to generate variant libraries, but they generally have significant drawbacks and limitations.  Twist Bioscience Site Saturation Variant Libraries leverage massively parallel oligonucleotide synthesis using a silicon-based DNA synthesis platform and extensive molecular biology expertise to systematically and precisely construct variant libraries. Twist libraries are NGS-verified to confirm that all desired variants are present in the correct ratios.