Simultaneous Analysis of Intact Human Insulin and Five Analogs in Human Plasma Using μElution SPE and a CORTECS UPLC Column
Simultaneous Analysis of Intact Human Insulin and Five Analogs in Human Plasma Using μElution SPE and a CORTECS UPLC Column
3 Nov 2015Insulin is perhaps one of the best known and earliest peptide therapeutics. Multiple long and fast-acting analogs have also been developed, and a patient may often be prescribed one of each for diabetes control. Quantification of biologics, such as insulins, has historically been performed using ligand binding assays (LBAs) such as ELISAS. LC/MS/MS, however, has certain advantages over LBAs, such as shorter development times, higher accuracy and precision, the ability to multiplex, no cross-reactivity, and the ability to readily distinguish between closely related insulins. This work provides a single, simple method for the simultaneous, direct quantification of intact human insulin and multiple insulin analogs in human plasma using mixed-mode solid-phase extraction (SPE) and a high-efficiency, solid-core particle column that contains a low-level positive surface charge.
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CORTECS® Columns
WatersUnleash the power of your laboratory's instrumentation. CORTECS® 1.6 µm and 2.7 µm solid-core columns deliver new levels of performance. CORTECS Columns are available in both reversed-phase and HILIC options, giving you the flexibility and power to develop methods for challenging separations even faster. With different particle characteristics and innovative charged surface modifications, CORTECS Columns are suitable for use in a wide variety of applications. CORTECS Columns offer particle sizes ideal for HPLC, UHPLC, and UPLC platforms, assuring a simple, seamless method transfer with consistent results across different particle sizes and column configurations. CORTECS 1.6 µm and 2.7 µm solid-core columns are suitable for use in a wide variety of applications. CORTECS Columns offer particle sizes ideal for HPLC, UHPLC, and UPLC platforms, assuring a simple, seamless method transfer with consistent results across different particle sizes and column configurations.
Oasis® µElution Plate
WatersThe Oasis® uElution plate format combined with the Oasis chemistries allow elution volumes as low as 25uL, eliminating the time consuming evaporation step, producing very clean extracts with a 5 to 10 fold increase in concentration.The Oasis® family of sample extraction products is designed to simplify and improve your sample preparation by combining the right sorbent chemistry, device format and methodology. Achieve robust, selective, and sensitive solid-phase extraction [SPE] methods without worrying about low recoveries caused by breakthrough, sorbent drying, pH limitations, and undesirable silanol activity.Oasis® SPE sorbents—covered by eight U.S. patents—are unique in their purity, reproducibility, stability, and retention characteristics. Today, they are the most widely used polymeric SPE products in bioanalytical laboratories.