Application Note: Reference Yeast and Human Whole Cell Protein Extracts for Mass Spectrometry Instrument Performance Monitoring and Method Development
9 July 2015
This application note, two protein extraction formats are described: pre-digested extracts, for monitoring instrument performance, and intact protein extracts, for optimization of sample preparation. To be compatible with mass spectrometry applications, extracts need to meet certain requirements, like freedom from detergents, high protein integrity and high reproducibility. Here, whole cell protein extracts from yeast and human cells that meet those criteria are described.