Application Note: Rapid Separation of Basic Drug Compounds Under Alkaline pH Conditions on pH-Stable, Hamilton PRP-C18
27 November 2014

Mobile phase pH is a powerful tool in methods development, particularly for separation of neutral forms of amines or other organic bases under alkaline conditions. In this application note, a generic, 5-min linear gradient was used to separate six basic drug compounds on a short (50 mm) PRP-C18 column.