Application Note: Peptide quantification in plasma using the Agilent 6495 Triple Quadrupole LC/MS coupled with the Agilent 1290 Infinity II LC System
1 October 2020

In translational research, increased throughput as well as increased multiplexing mass spectrometry using MRM-based LC/MS methods have become popular for targeted, bottom-up quantification of protein biomarkers. Researchers often target signature peptides from a limited number of proteins in large cohorts and monitor their expression levels during a specified time period, often leading to hundreds or thousands of biological samples. In such large-volume studies, high-throughput, robustness, and reproducibility are essential when deploying LC/MS methods. This application note demonstrates the quantitative analytical sensitivity of 99 signature peptides from 75 protein biomarkers in plasma using the 6495 triple quadrupole LC/MS system and AJS ionization source.

For Research Use Only. Not for use in diagnostic procedures.